Distinguished Women in Science: Professor Eranthie Weerapana, Boston College

Distinguished Women in Science: Professor Eranthie Weerapana, Boston College
Date
Mon May 20th 2019, 4:30 - 5:30pm
Location
Sapp Center Auditorium

Distinguished Women in Science: Professor Eranthie Weerapana, Boston College (Host: Bette Webster)

About the Seminar

"Chemical-proteomic strategies to investigate cysteine reactivity"

Cysteine residues are critical to the catalytic and regulatory functions of diverse proteins including proteases, oxidoreductases and kinases. Although the majority of catalytic cysteine residues within the human proteome are well annotated, the identity and endogenous functions of regulatory cysteines are poorly understood. These regulatory cysteines are often located distal to the catalytic or ligand-binding sites of proteins and typically regulate protein function through posttranslational modifications such as oxidation, nitrosation and lipidation. To identify and characterize regulatory cysteines, my lab has developed and utilized a suite of chemical-proteomic technologies that report on global changes in cysteine reactivity in vitro and in situ. Several key studies demonstrate the widespread applications of our cysteine reactivity-profiling platforms, including: (1) identifying sites of S-nitrosation in proteomes and characterizing two unannotated S-nitrosation-sensitive cysteine residues on HADH2 and CTSD; (2) developing caged cysteine-reactive probes to identify sites of cysteine oxidation directly in living cells during epidermal growth-factor (EGF) signaling; (3) enriching mitochondria to increase the number of identified mitochondrial cysteines by >10 fold over previous studies; (4) elucidating metal-binding sites on proteins, including cysteine residues involved in zinc and iron coordination; and, (5) characterizing cysteine-mediated protein activities that regulate lifespan through the insulin/IGF-1 signaling pathway. Together, these studies highlight the utility of our chemical-proteomic platforms for elucidating cysteine function in disparate biological systems.

About the Speaker

Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.